I have seen many authenticated framed items from JSA that were authenticated without removal from the frame. However, they are usually single signed items.
Not sure if anyone will authenticate the item with this many signatures and let's face it these are not everyday name autographs. Who knows if they have exemplars for all of these autographs.
Good luck with the authentication but I would not be surprised if they passed on authenticating them in or out of the frame.
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